UHPLC Columns (Micro/Cap/Nano)
Ultra-High Performance Liquid Chromatography (UHPLC)
SciSEP are pleased to introduce Microsil® 1.8µm particles in C18, C8 and C4 phases for ultra-high pressure liquid chromatography. Formulated by Dr Frank Yang, inventor of the packed-fused-silica capillary column, these sub-2µm particle columns are specifically optimised to give sharp symmetrical peaks, increased resolution, excellent reproducibility and high durability under rigorous column conditions.
Characteristics
| Column Characteristics |
Available in |
| LC/MS compatible |
1.8µm particle size, 120Å pore size |
| High Throughput |
C18, C8 and C4 phases |
| High Resolution |
Microbore (1.0 – 2.0mm column ID) |
| Low Sample Loss |
Nano (25, 50 and 75µm ID) |
| Max Pressure up to 15,000 psi |
Capillary (150 and 320µm ID) |
- Every Column is individually tested and certified to pass the highest column performance standards. (Test conditions: analysis of peptide standard and detection by MS).
- Column incorporates porous 2µm frits or built-in on-column end frits at each end to ensure packed bed stability and reproducibility.
- Capillary and nano columns feature reversible solvent flow path to prevent column plugging.
HPLC and UHPLC Column Comparison
| Compare HPLC and UHPLC |
HPLC |
UHPLC |
| Column Dimensions (mm x mm) |
250 x 4.6 |
100 x 2 |
| Flow Rate (mL/min) |
1 |
0.55 |
| Run Time (min) |
25 |
4 |
| Sample throughput |
200 hrs |
30 hrs |
| Throughput Increase % |
- |
500% |
| Mobile Phase Required |
12L |
1L |
Efficiency and throughput for analysis of 500 samples (Average MW 300). Isocratic (90% Aqueous, 10% ACN), 30 degree C.
Column Selection
Microbore UHPLC Columns – 1.0 & 2.0mm ID (120Å pore size)
| Phase |
Column Length (cm) |
1.0mm ID Part number |
2.0mm ID part number |
| C18 |
5 |
MM-5-C18SSS-1000-EU |
MM-5-C18SSS-2000-EU |
| C18 |
10 |
MM-10-C18SSS-1000-EU |
MM-10-C18SSS-2000-EU |
| C18 |
15 |
MM-15-C18SSS-1000-EU |
MM-15-C18SSS-2000-EU |
| C18 |
1cm trap cartridge & holder |
MM-1.0-C18SSS-1000-Trap |
MM-1.0-C18SSS-2000-Trap |
| C18 |
1cm trap cartridge |
MM-1.0-C18SSS-1000-Trap cartridge (pack of 3) |
MM-1.0-C18SSS-2000-Trap cartridge (pack of 3) |
| |
| C8 |
5 |
MM-5-C8SSS-1000-EU |
MM-5-C8SSS-2000-EU |
| C8 |
10 |
MM-10-C8SSS-1000-EU |
MM-10-C8SSS-2000-EU |
| C8 |
15 |
MM-15-C8SSS-1000-EU |
MM-15-C8SSS-2000-EU |
| C8 |
1cm trap cartridge & holder |
MM-1.0-C8SSS-1000-Trap |
MM-1.0-C8SSS-2000-Trap |
| C8 |
1cm trap cartridge |
MM-1.0-C8SSS-1000-Trap cartridge (pack of 3) |
MM-1.0-C8SSS-2000-Trap cartridge (pack of 3) |
| |
| C4 |
5 |
MM-5-C4SSS-1000-EU |
MM-5-C4SSS-2000-EU |
| C4 |
10 |
MM-10-C4SSS-1000-EU |
MM-10-C4SSS-2000-EU |
| C4 |
15 |
MM-15-C4SSS-1000-EU |
MM-15-C4SSS-2000-EU |
| C4 |
1cm trap cartridge & holder |
MM-1.0-C4SSS-1000-Trap |
MM-1.0-C4SSS-2000-Trap |
| C4 |
1cm trap cartridge |
MM-1.0-C4SSS-1000-Trap cartridge (pack of 3) |
MM-1.0-C4SSS-2000-Trap cartridge (pack of 3) |
Nano-UHPLC Columns – 25, 50 & 75µm ID (120Å pore size)
| Phase |
Column Length (cm) |
25µm ID part number |
50µm ID part number |
75µm ID part number |
| C18 |
5 |
MN-5-C18SSS-25-EU |
MN-5-C18SSS-50-EU |
MN-5-C18SSS-75-EU |
| C18 |
10 |
MN-10-C18SSS-25-EU |
MN-10-C18SSS-50-EU |
MN-10-C18SSS-75-EU |
| C18 |
15 |
MN-15-C18SSS-25-EU |
MN-15-C18SSS-50-EU |
MN-15-C18SSS-75-EU |
| C18 |
25 |
MN-25-C18SSS-25-EU |
MN-25-C18SSS-50-EU |
MN-25-C18SSS-75-EU |
| C18 |
35 |
MN-35-C18SSS-25-EU |
MN-35-C18SSS-50-EU |
MN-35-C18SSS-75-EU |
| C18 |
2.5 trap column |
MN-2.5-C18SSS-25-EU-Trap |
MN-2.5-C18SSS-50-EU-Trap |
MN-2.5-C18SSS-75-EU-Trap |
| |
| C8 |
5 |
MN-5-C8SSS-25-EU |
MN-5-C8SSS-50-EU |
MN-5-C8SSS-75-EU |
| C8 |
10 |
MN-10-C8SSS-25-EU |
MN-10-C8SSS-50-EU |
MN-10-C8SSS-75-EU |
| C8 |
15 |
MN-15-C8SSS-25-EU |
MN-15-C8SSS-50-EU |
MN-15-C8SSS-75-EU |
| C8 |
25 |
MN-25-C8SSS-25-EU |
MN-25-C8SSS-50-EU |
MN-25-C8SSS-75-EU |
| C8 |
35 |
MN-35-C8SSS-25-EU |
MN-35-C8SSS-50-EU |
MN-35-C8SSS-75-EU |
| C8 |
2.5 trap column |
MN-2.5-C8SSS-25-EU-Trap |
MN-2.5-C8SSS-50-EU-Trap |
MN-2.5-C8SSS-75-EU-Trap |
| |
| C4 |
5 |
MN-5-C4SSS-25-EU |
MN-5-C4SSS-50-EU |
MN-5-C4SSS-75-EU |
| C4 |
10 |
MN-10-C4SSS-25-EU |
MN-10-C4SSS-50-EU |
MN-10-C4SSS-75-EU |
| C4 |
15 |
MN-15-C4SSS-25-EU |
MN-15-C4SSS-50-EU |
MN-15-C4SSS-75-EU |
| C4 |
25 |
MN-25-C4SSS-25-EU |
MN-25-C4SSS-50-EU |
MN-25-C4SSS-75-EU |
| C4 |
35 |
MN-35-C4SSS-25-EU |
MN-35-C4SSS-50-EU |
MN-35-C4SSS-75-EU |
| C4 |
2.5 trap column |
MN-2.5-C4SSS-25-EU-Trap |
MN-2.5-C4SSS-50-EU-Trap |
MN-2.5-C4SSS-75-EU-Trap |
Capillary UHPLC Columns – 150 & 320µm ID (120Å pore size)
| Phase |
Column Length (cm) |
150µm ID Part number |
320µm ID part number |
| C18 |
5 |
MN-5-C18SSS-150-EU |
MC-5-C18SSS-320-EU |
| C18 |
10 |
MN-10-C18SSS-150-EU |
MC-10-C18SSS-320-EU |
| C18 |
15 |
MN-15-C18SSS-150-EU |
MC-15-C18SSS-320-EU |
| C18 |
25 |
MN-25-C18SSS-150-EU |
MC-25-C18SSS-320-EU |
| C18 |
35 |
MN-35-C18SSS-150-EU |
MC-35-C18SSS-320-EU |
| C18 |
2.5 trap column |
MN-2.5-C18SSS-150-EU-Trap |
MC-2.5-C18SSS-320-EU-Trap |
| |
| C8 |
5 |
MN-5-C8SSS-150-EU |
MC-5-C8SSS-320-EU |
| C8 |
10 |
MN-10-C8SSS-150-EU |
MC-10-C8SSS-320-EU |
| C8 |
15 |
MN-15-C8SSS-150-EU |
MC-15-C8SSS-320-EU |
| C8 |
25 |
MN-25-C8SSS-150-EU |
MC-25-C8SSS-320-EU |
| C8 |
35 |
MN-35-C8SSS-150-EU |
MC-35-C8SSS-320-EU |
| C8 |
2.5 trap column |
MN-2.5-C8SSS-150-EU-Trap |
MC-2.5-C8SSS-320-EU-Trap |
| |
| C4 |
5 |
MN-5-C4SSS-150-EU |
MC-5-C4SSS-320-EU |
| C4 |
10 |
MN-10-C4SSS-150-EU |
MC-10-C4SSS-320-EU |
| C4 |
15 |
MN-15-C4SSS-150-EU |
MC-15-C4SSS-320-EU |
| C4 |
25 |
MN-25-C4SSS-150-EU |
MC-25-C4SSS-320-EU |
| C4 |
35 |
MN-35-C4SSS-150-EU |
MC-35-C4SSS-320-EU |
| C4 |
2.5 trap column |
MN-2.5-C4SSS-150-EU-Trap |
MC-2.5-C4SSS-320-EU-Trap |
Product Data and Information
